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Description

The 488 nm laser was used for excitation of GFP, tagRFP was excited with the 561 nm laser

The protein was immunolocalized in the epidermis and parenchyma of plerocercoid, and in the epidermis, parenchyma, uterus and egg shell of adult worm

The organic phases were combined, washed twice with saturated brine, dried over anhydrous sodium sulfate, and purified to obtain a white solid product S1 (240.4 mg, purity 92%)